Nutrients drive transcriptional changes that maintain metabolic homeostasis but alter genome architecture in Microcystis

摘要: The cyanobacterium Microcystis aeruginosa is a globally distributed bloom-forming organism that degrades freshwater systems around the world. Factors drive its dispersion, diversification and success remain, however, poorly understood. To develop insight into cellular-level responses to nutrient drivers of eutrophication, RNA sequencing was coupled comprehensive metabolomics survey M. sp. NIES 843 grown in various nutrient-reduced conditions. Transcriptomes were generated for cultures nutrient-replete (with nitrate as nitrogen (N) source), nitrogen-reduced nitrate, urea or ammonium acting N sources) phosphate-reduced Extensive expression differences (up 696 genes urea-grown cells) relative control treatment observed, demonstrating chemical variant available cells affected transcriptional activity. Of particular note, high number transposase 81) significantly reproducibly up-regulated when on urea. Conversely, phosphorus (P) reduction resulted significant cessation transcription genes, indicating variation chemistry may influence transposases impact highly mosaic genomic architecture aeruginosa. Corresponding metabolomes showed comparably few between treatments, suggesting broad changes gene are required maintain metabolic homeostasis under reduction. combined observations provide novel extensive complex cellular interactions take place this important during variable conditions highlight potential unknown molecular mechanism blooms evolution.