Nitrile-converting enzymes as a tool to improve biocatalysis in organic synthesis: recent insights and promises

摘要: Nitrile-converting enzymes, including nitrilase and nitrile hydratase (NHase), have received increasing attention from researchers of industrial biocatalysis because their critical role as a tool in organic synthesis carboxylic acids amides nitriles. To date, these bioconversion approaches are considered one the most potential processes using resting cells or purified enzymes catalysts for production food additives, pharmaceutical, agrochemical precursors. This review focuses on distribution catalytic mechanism research nitrile-converting recent years. Molecular biology aspects to improve biocatalytic performance microbial NHase demonstrated. The process developments also discussed.