Self-assembly amphipathic peptides induce active enzyme aggregation that dramatically increases the operational stability of nitrilase

作者: Xiaofeng Yang , An Huang , Jizong Peng , Jufang Wang , Xiaoning Wang

DOI: 10.1039/C4RA11236A

关键词:

摘要: Oligomeric nitrilase was fused with an amphipathic self-assembly peptide 18A at the C-terminus and expressed in Escherichia coli. The fusion enzyme spontaneously assembled …

参考文章(46)
Patrick Mueller, Ksenia Egorova, Constantinos E. Vorgias, Effrosini Boutou, Harald Trauthwein, Stefan Verseck, Garabed Antranikian, Cloning, overexpression, and characterization of a thermoactive nitrilase from the hyperthermophilic archaeon Pyrococcus abyssi. Protein Expression and Purification. ,vol. 47, pp. 672- 681 ,(2006) , 10.1016/J.PEP.2006.01.006
Christoph Kiziak, Doris Conradt, Andreas Stolz, Ralf Mattes, Joachim Klein, Nitrilase from Pseudomonas fluorescens EBC191: cloning and heterologous expression of the gene and biochemical characterization of the recombinant enzyme. Microbiology. ,vol. 151, pp. 3639- 3648 ,(2005) , 10.1099/MIC.0.28246-0
Zhanglin Lin, Bihong Zhou, Wei Wu, Lei Xing, Qing Zhao, Self-assembling amphipathic alpha-helical peptides induce the formation of active protein aggregates in vivo Faraday Discussions. ,vol. 166, pp. 243- 256 ,(2013) , 10.1039/C3FD00068K
Lawrence J. Mersinger, Eugenia C. Hann, Frederick B. Cooling, John E. Gavagan, Arie Ben-Bassat, Shijun Wu, Kelly L. Petrillo, Mark S. Payne, Robert DiCosimo, Production of Acrylamide using Alginate‐Immobilized E. coli Expressing Comamonas testosteroni 5‐MGAM‐4D Nitrile Hydratase Advanced Synthesis & Catalysis. ,vol. 347, pp. 1125- 1131 ,(2005) , 10.1002/ADSC.200505039
Yi Liu, Wenjing Cui, Zhongmei Liu, Youtian Cui, Yuanyuan Xia, Michihiko Kobayashi, Zhemin Zhou, Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide Journal of Bioscience and Bioengineering. ,vol. 118, pp. 249- 252 ,(2014) , 10.1016/J.JBIOSC.2014.02.015
David Costes, Gints Rotčnkovs, Ernst Wehtje, Patrick Adlercreutz, Stability and Stabilization of Hydroxynitrile Lyase in Organic Solvents Biocatalysis and Biotransformation. ,vol. 19, pp. 119- 130 ,(2001) , 10.3109/10242420109003640
Xinyao Lu, Song Liu, Dongxu Zhang, Xiaoman Zhou, Miao Wang, Yi Liu, Jing Wu, Guocheng Du, Jian Chen, Enhanced thermal stability and specific activity of Pseudomonas aeruginosa lipoxygenase by fusing with self-assembling amphipathic peptides Applied Microbiology and Biotechnology. ,vol. 97, pp. 9419- 9427 ,(2013) , 10.1007/S00253-013-4751-Y
Grace DeSantis, Kelvin Wong, Bob Farwell, Kelly Chatman, Zoulin Zhu, Geoff Tomlinson, Hongjun Huang, Xuqiu Tan, Lisa Bibbs, Pei Chen, Keith Kretz, Mark J. Burk, Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM). Journal of the American Chemical Society. ,vol. 125, pp. 11476- 11477 ,(2003) , 10.1021/JA035742H
Kerstin Steiner, Helmut Schwab, Recent advances in rational approaches for enzyme engineering Computational and structural biotechnology journal. ,vol. 2, pp. 1- 12 ,(2012) , 10.5936/CSBJ.201209010