Enhanced thermal stability and specific activity of Pseudomonas aeruginosa lipoxygenase by fusing with self-assembling amphipathic peptides
作者： Xinyao Lu , Song Liu , Dongxu Zhang , Xiaoman Zhou , Miao Wang
DOI: 10.1007/S00253-013-4751-Y
关键词:
摘要: Self-assembling amphipathic peptides (SAPs) are a category of peptides that have unique sequences with alternating hydrophobic and hydrophilic residues that can spontaneously assemble into ordered nanostructures. In this study, we investigated the potential of fusion technique with SAPs to improve the thermal stability of lipoxygenase (LOX) from Pseudomonas aeruginosa. Six SAPs were individually fused to the N terminus of the LOX that resulted to the SAP–LOX fusions with approximately 2.3-to 4.5-fold enhanced thermal …
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