Freezing of Isolated Thylakoid Membranes in Complex Media. VII. The Effect of Bovine Serum Albumin
作者： Kurt A. Santarius
DOI: 10.1016/S0015-3796(11)80119-8
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摘要: Summary THe cryoprotective properties of bovine serum albumin (BSA) were investigated with thylakoid membranes isolated from spinach leaves ( Spinacia oleracea L. cv. Monatol). The frozen in a complex salt medium containing the predominant inorganic electrolytes chloroplast stroma. Under mild freezing conditions, only partial stabilization PSII-dependent photosynthetic activities but efficient cryopreservation PSI-mediated photophosphorylation took place presence BSA. lower temperature, less protein was necessary for optimum protection light-induced proton uptake and ATP synthesis. At higher initial BSA concentrations or more severe freezing, i.e. when concentration residual unfrozen fraction exceeded critical limit, rapid inactivation occurred parallel ice crystal formation. This damage due to an increase permeability membranes, while capacity linear whole-chain electron transport remained unaffected even rose relative membranes. effects solutes on can be explained part by colligative action, each solute acts different membrane sites reduces others liquid. In addition, influenced quantity formed, final volume solution, this duration time exposure given temperature. Extremely high thylakoids system caused regardless liquid When kept at 0 °C, effectively stabilized cyclic slightly reduced progressive flow.
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sci-hub.se 参考文章(25)
Kurt A. Santarius, Christoph Giersch, Cryopreservation of spinach chloroplast membranes by low-molecular-weight carbohydrates Cryobiology. ,vol. 20, pp. 90- 99 ,(1983) , 10.1016/0011-2240(83)90063-9
Kurt A. Santarius, The effect of freezing on thylakoid membranes in the presence of organic acids. Plant Physiology. ,vol. 48, pp. 156- 162 ,(1971) , 10.1104/PP.48.2.156
S. Grafflage, G. H. Krause, Simulation of in situ freezing damage of the photosynthetic apparatus by freezing in vitro of thylakoids suspended in complex media Planta. ,vol. 168, pp. 67- 76 ,(1986) , 10.1007/BF00407011
Annelori Rosas, Miren Alberdi, Michel Delseny, Meza-Basso Luis, A cryoprotective polypeptide isolated from Nothofagus dombeyi seedlings Phytochemistry. ,vol. 25, pp. 2497- 2500 ,(1986) , 10.1016/S0031-9422(00)84495-7
K. A. Santarius, Freezing of isolated thylakoid membranes in complex media : III. Differences in the pattern of inactivation of photosynthetic reactions. Planta. ,vol. 168, pp. 281- 286 ,(1986) , 10.1007/BF00402975
Albert J. Robertson, Lawrence V. Gusta, Martin J. T. Reaney, Masaya Ishikawa, Identification of Proteins Correlated with Increased Freezing Tolerance in Bromegrass (Bromus inermis Leyss. cv Manchar) Cell Cultures Plant Physiology. ,vol. 86, pp. 344- 347 ,(1988) , 10.1104/PP.86.2.344
Sarah J. Gilmour, Ravindra K. Hajela, Michael F. Thomashow, Cold Acclimation in Arabidopsis thaliana Plant Physiology. ,vol. 87, pp. 745- 750 ,(1988) , 10.1104/PP.87.3.745
Sirpa Kurkela, Marianne Franck, Pekka Heino, Viola L�ng, E. Tapio Palva, Cold induced gene expression in Arabidopsis thaliana L. Plant Cell Reports. ,vol. 7, pp. 495- 498 ,(1988) , 10.1007/BF00272740
H.G. Volger, U. Heber, Cryoprotective leaf proteins. Biochimica et Biophysica Acta. ,vol. 412, pp. 335- 349 ,(1975) , 10.1016/0005-2795(75)90048-3
U. Heber, M. Kempfle, Proteine als Schutzstoffe gegenüber dem Gefriertod der Zelle Zeitschrift für Naturforschung B. ,vol. 25, pp. 834- 842 ,(1970) , 10.1515/ZNB-1970-0812