Investigation of allogeneic mesenchymal stem cell-based alveolar bone formation: preliminary findings.

摘要: This study was designed to evaluate mesenchymal stem cell (MSC)-based alveolar bone regeneration in a canine saddle defect model. MSCs were loaded onto hydroxyapatite/tricalcium phosphate (HA/TCP) matrices. Scanning electron microscopic (SEM) evaluation demonstrated greater than 75% MSC coverage of the HA/TCP porous surface prior placement regardless donor. Matrices, 6 mm x 20 mm, with and without cells, implanted for 4 9 weeks, then removed histological formation. Cell-free control matrices compared MSC-loaded post implantation. Histomorphometrical analysis showed that equivalent amounts new formed within pores autologous or from an unrelated Bone formation cell-free less extensive. There no histologic evidence immunological response MSCs. Surprisingly, allogeneic implantation also failed provoke immune response. Analysis circulating antibody levels against supported hypothesis neither nor induced systemic by host. dye-labelled sections confirmed persisted implants throughout course experiment. At labelled cells present lacunae newly bone. We conclude have capacity regenerate craniofacial defects.