Induction of Angiogenesis by Matrigel Coating of VEGF-Loaded PEG/PCL-Based Hydrogel Scaffolds for hBMSC Transplantation
作者： Yeon Joo Jung , Kyung-Chul Kim , Jun-Young Heo , Kaipeng Jing , Kyung Eun Lee
DOI: 10.14348/MOLCELLS.2015.0142
关键词:
摘要: hBMSCs are multipotent cells that useful for tissue regeneration to treat degenerative diseases and others their differentiation ability into chondrocytes, osteoblasts, adipocytes, hepatocytes neuronal cells. In this study, biodegradable elastic hydrogels consisting of hydrophilic poly(ethylene glycol) (PEG) hydrophobic poly(e-caprolactone) (PCL) scaffolds were evaluated engineering because its biocompatibility the control release bioactive peptides. The primary cultured from human bone marrow confirmed as hBMSC by immunohistochemical analysis. Mesenchymal stem cell markers (collagen type I, fibronectin, CD54, integrin1β, Hu protein) shown be positive, while hematopoietic (CD14 CD45) negative. Three different hydrogel with block compositions (PEG:PCL=6:14 14:6 weight) fabricated using salt leaching method. expanded, seeded on scaffolds, up 8 days under static conditions in Iscove's Modified Dulbecco's Media (IMDM). growth MSCs PEG/PCL= 6/14 was faster than others. addition, morphology seemed normal no cytotoxicity found. coating vascular endothelial factor (VEGF) containing scaffold Matrigel slowed down VEGF vitro promoted angiogenesis when transplanted BALB/c nude mice. These results suggest can supported a biode gradable effective growth, enhance coating.
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