Polyurethanes as Supports for Human Retinal Pigment Epithelium Cell Growth
作者： Gisele R Da Silva , Da SC Armando , Juliana B Saliba , Marianne Berdugo , Brigitte T Goldenberg
关键词: Retina 、 Anatomy 、 Biocompatibility 、 Cell adhesion 、 Chemistry 、 Adhesion 、 Ethylene glycol 、 Biophysics 、 Polyurethane 、 Retinal pigment epithelium 、 Isophorone diisocyanate
摘要: Purpose The transplant of retinal pigment epithelium (RPE) cells on supports may well be an effective therapeutic approach to improve the visual results patients with age-related macular degeneration. In this study, two biodegradable polyurethanes were investigated as for human RPE (ARPE-19). Methods Polyurethane aqueous dispersions based poly(caprolactone) and/or poly(ethylene glycol) soft segments, and isophorone diisocyanate hydrazine hard segments prepared. films produced by casting allowing them dry at room temperature one week. ARPE-19 seeded onto polyurethane they in vitro adhesion, proliferation, uniform distribution differentiated cells. Additionally, vivo ocular biocompatibility was evaluated. Results adhered proliferated supports, thus establishing cell-PUD surface interactions. Upon confluence, formed organized monolayer, exhibited a polygonal appearance, displayed actin filaments which ran along upper cytoplasm. At 15 days seeding, occluding expression confirmed between adjacent cells, representing barrier functionality epithelial polymeric surfaces establishment cell-cell from study indicated that high degree short-term intraocular biocompatibility. Conclusions Biodegradable display proper mechanical properties easy transscleral-driven subretinal implantation can considered biocompatible functional monolayer.
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