Glioma cells under hypoxic conditions block the brain microvascular endothelial cell death induced by serum starvation.
作者： Yoshifumi Ueda , Takao Nakagawa , Toshihiko Kubota , Kazunori Ido , Kazufumi Sato
DOI: 10.1111/J.1471-4159.2005.03343.X
关键词:
摘要: Angiogenesis is one of essential components for the growth neoplasms, including malignant gliomas. However, tumor vascularization often poorly organized and marginally functional due to structural abnormalities, inducing regional or temporal hypoxic conditions nutritional shortages in tissues. We investigated how during angiogenesis migrating endothelial cells survive these reduced conditions. Human brain microvascular (HBMECs) underwent apoptosis necrosis after serum withdrawal. This cell death was blocked by recombinant VEGF protein culture medium U251 glioma exposed hypoxia (H-CM). Hypoxic treatment increased vascular factor (VEGF) alpha (TNF-alpha) expression cells. H-CM activated nuclear factor-kappaB (NFkappaB) gene antiapoptotic factors Bcl-2, Bcl-X(L), survivin X-chromosome-linked inhibitor (XIAP) The survival activity abolished two kinds inhibitors {Cyclopeptidic a receptor tyrosine kinase (4-[(4'-chloro-2'-fluoro) phenylamino]-6, 7-dimethoxyquinazoline)} NFkappaB (ALLN BAY 11-7082). These did not block activation induced On contrary, TNF-alpha antagonist WP9QY enhanced under serum-starved Taken together, our data suggest that both secretion from are necessary as they increase genes starvation. pathways may be mechanisms which maintained
sci-hub.se 参考文章(56)
P. Vaupel, F. Kallinowski, P. Okunieff, Blood flow, oxygen consumption and tissue oxygenation of human tumors. Advances in Experimental Medicine and Biology. ,vol. 277, pp. 895- 905 ,(1990) , 10.1007/978-1-4684-8181-5_103
Bernard Robaye, Jacques Emile Dumont, Paul Galand, Walter Fiers, R Mosselmans, Tumor necrosis factor induces apoptosis (programmed cell death) in normal endothelial cells in vitro. American Journal of Pathology. ,vol. 138, pp. 447- 453 ,(1991)
S D Prionas, L F Fajardo, J Kowalski, A C Allison, H H Kwan, Dual role of tumor necrosis factor-alpha in angiogenesis. American Journal of Pathology. ,vol. 140, pp. 539- 544 ,(1992)
Peter J. Polverini, Robert M. Strieter, David J. Mooney, Jacques E. Nör, Joan Christensen, Martin Peters, Jianguo Liu, Up-Regulation of Bcl-2 in microvascular endothelial cells enhances intratumoral angiogenesis and accelerates tumor growth. Cancer Research. ,vol. 61, pp. 2183- 2188 ,(2001)
M Kuwano, J Van Damme, T Matsuda, K Kohno, M Mawatari, K Asoh, H Mizoguchi, H G Welgus, Effects of tumor necrosis factor and epidermal growth factor on cell morphology, cell surface receptors, and the production of tissue inhibitor of metalloproteinases and IL-6 in human microvascular endothelial cells. Journal of Immunology. ,vol. 143, pp. 1619- 1627 ,(1989)
Xiaolong Hu, Esther Yee, John M. Harlan, Fred Wong, Aly Karsan, Lipopolysaccharide induces the antiapoptotic molecules, A1 and A20, in microvascular endothelial cells. Blood. ,vol. 92, pp. 2759- 2765 ,(1998) , 10.1182/BLOOD.V92.8.2759
A W Opipari, H.M. Hu, R Yabkowitz, V.M. Dixit, The A20 zinc finger protein protects cells from tumor necrosis factor cytotoxicity. Journal of Biological Chemistry. ,vol. 267, pp. 12424- 12427 ,(1992) , 10.1016/S0021-9258(18)42292-2
Marcia Regina Machein, Karl Heinz Plate, VEGF in brain tumors. Journal of Neuro-oncology. ,vol. 50, pp. 109- 120 ,(2000) , 10.1023/A:1006416003964
Russell Ross, Elaine W. Raines, Bodo Levkau, Marta Scatena, Cecilia M. Giachelli, Apoptosis overrides survival signals through a caspase-mediated dominant-negative NF-kappa B loop. Nature Cell Biology. ,vol. 1, pp. 227- 233 ,(1999) , 10.1038/12050
Motohiro Nomura, Sho-ichi Yamagishi, Shin-ichi Harada, Yasuhiko Hayashi, Tetsumori Yamashima, Junkoh Yamashita, Hiroshi Yamamoto, Possible participation of autocrine and paracrine vascular endothelial growth factors in hypoxia-induced proliferation of endothelial cells and pericytes. Journal of Biological Chemistry. ,vol. 270, pp. 28316- 28324 ,(1995) , 10.1074/JBC.270.47.28316