Involvement of phosphatidylinositol-3 kinase–Akt and nuclear factor kappa-B pathways in the effect of frutalin on human lymphocyte

摘要: Abstract The mechanisms involved in the mitogenic effect of lectins are not fully understood and thought to involve a cascade intracellular signals related T cell receptor activation. This study shows that frutalin, α- d -galactose-binding lectin from Artocarpus incisa seeds, is potent activator human lymphocytes. inhibited by -galactose PI3K inhibitors, accompanied an increase IL-2 expression PI3K-dependent gene protein synthesis. Frutalin also induces Akt-phosphorylation activates NF-κB, inducing its translocation cytosol nucleus. Both effects blocked presence or inhibitors. In summary, interacting with -galactose, signaling pathways TCR, thereby triggers PI3K/Akt NF-κB pathway, which modulates proliferation, synthesis IL-2R expression. might be useful tool following activation link upstream downstream events.