Influence of trace elements supplementation on the production of recombinant frutalin by Pichia pastoris KM71H in fed-batch process
作者： Marcela Wanderley , Carla Oliveira , Danyelly Bruneska , Lucília Domingues , José Lima Filho
DOI: 10.2478/S11696-013-0363-3
关键词:
摘要: Frutalin, a galactose-specific lectin used to detect specific tumour markers, is protein with low expression level in breadfruit. In the present study, fed-batch fermentation stirred tank bioreactor was as strategy enhance production by recombinant Pichia pastoris KM71H. By using this process, of frutalin 4-fold higher than value obtained shaker flasks batch assays. Supplementation medium trace elements (Pichia minerals, PTM) also evaluated order stimulate protein. The addition PTM minimum afforded 13.4 mg L−1, which 2.5-fold that achieved from culture without supplementation. These results are significant development strategies improve may broaden its application cancer diagnosis.
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Philippe Baumgartner, Karen Harper, Romaan J.M. Raemaekers, Alain Durieux, Angharad M.R. Gatehouse, Howard V. Davies, Mark A. Taylor, Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris Biotechnology Letters. ,vol. 25, pp. 1281- 1285 ,(2003) , 10.1023/A:1025007901322
Romaan J. M. Raemaekers, Laura de Muro, John A. Gatehouse, Anthony P. Fordham-Skelton, Functional phytohemagglutinin (PHA) and Galanthus nivalis agglutinin (GNA) expressed in Pichia pastoris correct N-terminal processing and secretion of heterologous proteins expressed using the PHA-E signal peptide. FEBS Journal. ,vol. 265, pp. 394- 403 ,(1999) , 10.1046/J.1432-1327.1999.00749.X
Aline C. Brando-Lima, Roberta F. Saldanha-Gama, Cristiane Ribeiro Pereira, Christina Gaspar Villela, André Luiz Franco Sampaio, Ana C.O. Monteiro-Moreira, Maria das Graças M.O. Henriques, Renato A. Moreira, Christina Barja-Fidalgo, Involvement of phosphatidylinositol-3 kinase–Akt and nuclear factor kappa-B pathways in the effect of frutalin on human lymphocyte International Immunopharmacology. ,vol. 6, pp. 465- 472 ,(2006) , 10.1016/J.INTIMP.2005.09.008
Nausicaä Lannoo, Wouter Vervecken, Paul Proost, Pierre Rougé, Els J.M. Van Damme, Expression of the nucleocytoplasmic tobacco lectin in the yeast Pichia pastoris. Protein Expression and Purification. ,vol. 53, pp. 275- 282 ,(2007) , 10.1016/J.PEP.2007.01.007
Koh AMANO, Mitsuyo TAKASE, Akikazu ANDO, Yoshiho NAGATA, Production of Functional Lectin in Pichia pastoris Directed by Cloned cDNA from Aleuria aurantia Bioscience, Biotechnology, and Biochemistry. ,vol. 67, pp. 2277- 2279 ,(2003) , 10.1271/BBB.67.2277
Jingli Xie, Qingwei Zhou, Peng Du, Renbao Gan, Qin Ye, Use of different carbon sources in cultivation of recombinant Pichia pastoris for angiostatin production Enzyme and Microbial Technology. ,vol. 36, pp. 210- 216 ,(2005) , 10.1016/J.ENZMICTEC.2004.06.010
Haifeng Hang, Xinhao Ye, Meijin Guo, Ju Chu, Yingping Zhuang, Ming Zhang, Siliang Zhang, A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate Enzyme and Microbial Technology. ,vol. 44, pp. 185- 188 ,(2009) , 10.1016/J.ENZMICTEC.2008.12.002
Philippe Baumgartner, Romaan J.M Raemaekers, Alain Durieux, Angharad Gatehouse, Howard Davies, Mark Taylor, Large-scale production, purification, and characterisation of recombinant Phaseolus vulgaris phytohemagglutinin E-form expressed in the methylotrophic yeast Pichia pastoris Protein Expression and Purification. ,vol. 26, pp. 394- 405 ,(2002) , 10.1016/S1046-5928(02)00555-7
Willy J Peumans, Soma Roy, Annick Barre, Pierre Rouge, Fred van Leuven, Els J.M van Damme, Elderberry (Sambucus nigra
) contains truncated Neu5Ac(α-2,6)Gal/GalNAc-binding type 2 ribosome-inactivating proteins FEBS Letters. ,vol. 425, pp. 35- 39 ,(1998) , 10.1016/S0014-5793(98)00193-8
Naoto Iijima, Koh Amano, Akikazu Ando, Yoshiho Nagata, Production of fruiting-body lectins of Pleurotus cornucopiae in methylotrophic yeast Pichia pastoris. Journal of Bioscience and Bioengineering. ,vol. 95, pp. 416- 418 ,(2003) , 10.1016/S1389-1723(03)80079-8