Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris
作者： Philippe Baumgartner , Karen Harper , Romaan J.M. Raemaekers , Alain Durieux , Angharad M.R. Gatehouse
关键词: Peptide sequence 、 Biology 、 Biochemistry 、 Lectin 、 Yeast 、 Pichia pastoris 、 Recombinant DNA 、 Gene 、 Galanthus nivalis 、 Agglutinin
摘要: The gene coding for agglutinin from Galanthus nivalis (GNA) was expressed in, and secreted by, the methylotrophic yeast, Pichia pastoris. Transformants of P. pastoris were selected a process to produce purify gram quantities recombinant GNA developed. at approximately 80 mg l−1 200 l scale purified 95% homogeneity using hydrophobic interaction chromatography. protein similar synthesised in plant with respect structure biological activity.
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