Effect of insecticidal fusion proteins containing spider toxins targeting sodium and calcium ion channels on pyrethroid-resistant strains of peach-potato aphid (Myzus persicae).
作者： Sheng Yang , Elaine Fitches , Prashant Pyati , John A Gatehouse
DOI: 10.1002/PS.3872
关键词: Myzus persicae 、 Botany 、 Voltage-dependent calcium channel 、 Fusion protein 、 Toxin 、 Voltage-gated ion channel 、 Pyrethroid 、 Wild type 、 Biochemistry 、 Biology 、 Spider toxin
摘要: BACKGROUND: The recombinant fusion proteins Pl1a/GNA and Hv1a/GNA contain the spider venom peptides δ-amaurobitoxin-PI1a or ω-hexatoxin-Hv1a respectively, linked to snowdrop lectin (GNA). Pl1a targets receptor site 4 of insect voltage-gated sodium channels (NaCh), while Hv1a calcium channels. Insecticide-resistant strains peach-potato aphid (Myzus persicae) mutations in NaCh. pyrethroid-resistant kdr (794J) super-kdr (UKO) at residues L1014 M918 channel α-subunit kdr + super-kdr strain (4824J), insensitive pyrethroids, contains both M918. RESULTS: have estimated LC50 values 0.35 0.19 mg mL−1 when fed wild-type M. persicae. For insecticide-resistant aphids, for protein increased by 2–6-fold, correlating with pyrethroid resistance (wild type < strains). In contrast, showed limited correlation resistance. CONCLUSION: Mutations aphids also protect against a containing sodium-channel-specific toxin, spite differences ligand–channel interactions, but do not confer targeting use differing could play role managing pesticide resistance.
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