Identification of stable housekeeping genes in response to ionizing radiation in cancer research.
作者： Gopal Iyer , Albert R. Wang , Sean R. Brennan , Shay Bourgeois , Eric Armstrong
DOI: 10.1038/SREP43763
关键词:
摘要: Housekeeping genes (HKGs) are essential for basic maintenance of a variety cellular processes. They ideally maintain uniform expression independent experimental conditions. However, the effects ionizing radiation (IR) on HKG is unclear. Statistical algorithms, geNorm and Normfinder were used estimating stability HKGs as raw quantification cycle (Cq) values not reliable factor normalization. Head neck, non-small lung pancreas cells exposed to 2, 4 6 Gy IR doses fourteen was measured at 5 min 48 h post-irradiation within given tissue. Paired single cell line analyses under these conditions identified TATA-Box Binding Protein (TBP) Importin 8 (IPO8) be stable in cancer. In addition two genes, Ubiquitin C (UBC) head neck cancer Transferrin receptor (TFRC) β-Glucuronidase (GUSB) pancreatic well. summary we present resource top ranked five their transcriptional behavior commonly model lines suggest use multiple treatment metric quantifying gene expression.
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