Quantification and Modeling of Plasmid Mobilization on Seeds and Roots

摘要: Mobilization frequencies of the nonconjugative plasmid pMON5003 were quantified using Escherichia coli TB1(pRK2013) as donor a helper plasmid, E. M182 (pMON5003) and Pseudomonas fluorescens recipient. Initial mating experiments conducted in nutrient minimal salts media pea seed exudates. rates higher during early stationary growth donors, helpers, recipients. Numbers transconjugants biparental matings when donors contained both conjugative plasmids, versus tri-parental matings. A mathematical model was developed to predict transfer rate parameter (δ), estimating proportion which is mobilized. Values δ ranged from 8 × 10−3 7.9 10−1. Transfer for P. on seeds roots determined. Transconjugants (P. 2-79 (pMON5003)) isolated seeds, roots, soil, but mobilization lower than liquid media.