Limited evolution but increasing trends of primary non-nucleoside reverse transcriptase inhibitor resistance mutations in therapy-naive HIV-1-infected individuals in India.

摘要: Background After the rapid scale-up of antiretroviral therapy (ART) in resource-limited settings, surveillance primary drug resistance mutations (DRMs) among ART-naive individuals has important public health benefits. Although a highly successful national ART programme initiated by Government India exists, data on prevalence DRMs is scarce. The objective study to estimate prevalence, pattern and spectrum population-based therapy-naive HIV-1-infected using clinical strains database sequences from seven HIV prevalent states India. Methods Drug genotyping was performed either plasma RNA or whole-blood genomic DNA validated in-house method 170 HIV-1-positive individuals. An additional 679 database-derived four other were included analysis. WHO-recommended list (SDRM_2009) for nucleoside reverse transcriptase inhibitors (NRTIs) non-nucleoside (NNRTIs) used interpretation DRMs. Trends before after rollout studied. Results overall 2.6% selected when isolates as well combined. Common T69D D67N (NRTI mutations), L100I, K101E, K103N Y181C (NNRTI mutations). There significant increase NNRTI over time. Conclusions DRM this low. However, an increasing trend been observed during past decade. Establishment threshold will be useful understanding further trends transmitted resistance.