Characterization of protein immobilization on nanoporous gold using atomic force microscopy and scanning electron microscopy

摘要: Nanoporous gold (NPG), made by dealloying low carat alloys, is a relatively new nanomaterial finding application in catalysis, sensing, and as support for biomolecules. NPG has attracted considerable interest due to its open bicontinuous structure, high surface-to-volume ratio, tunable porosity, chemical stability biocompatibility. also the attractive feature of being able be modified self-assembled monolayers. Here we use scanning electron microscopy (SEM) atomic force (AFM) characterize highly efficient approach protein immobilization on using N-hydroxysuccinimide (NHS) ester functionalized monolayers with pore sizes range tens nanometres. Comparison coupling under static versus flow conditions suggests that BSA (Bovine Serum Albumin) IgG (Immunoglobulin G) can only immobilized onto interior surfaces free standing monoliths good coverage conditions. AFM used examine both exterior NPG. Access surface imaging achieved special procedure cleaving rough comparative study. In principle, general described should applicable many enzymes, proteins complexes since functional groups present are controllable.