DNA ligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair.
作者： Ismail Abdou , Guy G. Poirier , Michael J. Hendzel , Michael Weinfeld
DOI: 10.1093/NAR/GKU1307
关键词:
摘要: In the current model of DNA SSBR, PARP1 is regarded as sensor single-strand breaks (SSBs). However, biochemical studies have implicated LIG3 another possible SSB sensor. Using a laser micro-irradiation protocol that predominantly generates SSBs, we were able to demonstrate dispensable for accumulation different break repair (SSBR) proteins at sites damage in live cells. Furthermore, show cells first time plays role mediating SSBR XRCC1 and PNKP damage. Importantly, did not require BRCT domain-mediated interaction with XRCC1. We N-terminal ZnF domain key enzyme's sensing function. Finally, provide cellular evidence acts caused by topoisomerase I inhibitor, irinotecan. Our results support existence second damage-sensing mechanism involving detection nicks genome LIG3.
oup.com
scienceopen.com
paperity.org
oxfordjournals.org参考文章(42)
Momchil D Vodenicharov, Frédéric R Sallmann, Masahiko S Satoh, Guy G Poirier, Base excision repair is efficient in cells lacking poly(ADP-ribose) polymerase 1 Nucleic Acids Research. ,vol. 28, pp. 3887- 3896 ,(2000) , 10.1093/NAR/28.20.3887
Maria Malanga, Felix R Althaus, The role of poly(ADP-ribose) in the DNA damage signaling network Biochemistry and Cell Biology. ,vol. 83, pp. 354- 364 ,(2005) , 10.1139/O05-038
Cecilia E Ström, Fredrik Johansson, Mathias Uhlen, Cristina Al-Khalili Szigyarto, Klaus Erixon, Thomas Helleday, Poly (ADP-ribose) polymerase (PARP) is not involved in base excision repair but PARP inhibition traps a single-strand intermediate Nucleic Acids Research. ,vol. 39, pp. 3166- 3175 ,(2011) , 10.1093/NAR/GKQ1241
I. D. Odell, J.-E. Barbour, D. L. Murphy, J. A. Della-Maria, J. B. Sweasy, A. E. Tomkinson, S. S. Wallace, D. S. Pederson, Nucleosome Disruption by DNA Ligase III-XRCC1 Promotes Efficient Base Excision Repair Molecular and Cellular Biology. ,vol. 31, pp. 4623- 4632 ,(2011) , 10.1128/MCB.05715-11
C. Dinant, M. de Jager, J. Essers, W. A. van Cappellen, R. Kanaar, A. B. Houtsmuller, W. Vermeulen, Activation of multiple DNA repair pathways by sub-nuclear damage induction methods. Journal of Cell Science. ,vol. 120, pp. 2731- 2740 ,(2007) , 10.1242/JCS.004523
K. W. Caldecott, S. Aoufouchi, P. Johnson, S. Shall, XRCC1 Polypeptide Interacts with DNA Polymerase β and Possibly Poly (ADP-Ribose) Polymerase, and DNA Ligase III Is a Novel Molecular ‘Nick-Sensor’ In Vitro Nucleic Acids Research. ,vol. 24, pp. 4387- 4394 ,(1996) , 10.1093/NAR/24.22.4387
S. Petrucco, Sensing DNA damage by PARP-like fingers Nucleic Acids Research. ,vol. 31, pp. 6689- 6699 ,(2003) , 10.1093/NAR/GKG890
JG Mabley, P Jagtap, Mauro Perretti, Stephen J Getting, Andrew L Salzman, L Virag, E Szabo, FG Soriano, L Liaudet, GE Abdelkarim, G Hasko, A Marton, GJ Southan, Csaba Szabo, Anti-inflammatory effects of a novel, potent inhibitor of poly (ADP-ribose) polymerase. Inflammation Research. ,vol. 50, pp. 561- 569 ,(2001) , 10.1007/PL00000234
Anna Campalans, Thierry Kortulewski, Rachel Amouroux, Hervé Menoni, Wim Vermeulen, J. Pablo Radicella, Distinct spatiotemporal patterns and PARP dependence of XRCC1 recruitment to single-strand break and base excision repair Nucleic Acids Research. ,vol. 41, pp. 3115- 3129 ,(2013) , 10.1093/NAR/GKT025
Zachary B. Mackey, Claude Niedergang, Josiane Ménissier-de Murcia, John Leppard, Karin Au, Jingwen Chen, Gilbert de Murcia, Alan E. Tomkinson, DNA Ligase III Is Recruited to DNA Strand Breaks by a Zinc Finger Motif Homologous to That of Poly(ADP-ribose) Polymerase IDENTIFICATION OF TWO FUNCTIONALLY DISTINCT DNA BINDING REGIONS WITHIN DNA LIGASE III Journal of Biological Chemistry. ,vol. 274, pp. 21679- 21687 ,(1999) , 10.1074/JBC.274.31.21679