Characterization of slow-growing root nodule bacteria from Inga oerstediana in organic coffee agroecosystems in Chiapas, Mexico

摘要: Abstract The leguminous tree genus Inga is thought to be critical for providing N certified organic coffee shrubs that commonly grow beneath its shade in the state of Chiapas, Mexico. Organic certification prohibits use synthetic fertilizers, necessitating understanding Inga–rhizobia symbiosis. objectives this study were to: (1) establish a collection isolates from sp. nodules found on farms Mexico; (2) phenotypic and genotypic approaches characterize these isolates, (3) examine cross-inoculation patterns strains isolated with other species N2-fixing trees. Eighty-three root oerstediana, pavoniana an unknown Inga. Aside I. pavoniana, demonstrate characteristics consistent Bradyrhizobium. Dendrograms generated using phylogenetic traits showed exhibit significant diversity, even among originating same farm. Strains 95% similar, displayed strikingly different species. Cross-inoculation experiments used three (cowpea miscellany CB756, oerstediana sp.) as inoculants edulis, fuellii, Acacia mangium, koa, Vigna unguculata Gliricidia sepium. All formed containing leghaemoglobin V. unguculata, while cowpea feulli but not A. mangium G. koa when inoculated by strain CB756 sp., did contain leghaemoglobin. One two-way ANOVA results no mean biomass seedlings was significantly greater than −N control each sepium, (p