Butylated hydroxytoluene induces hyperactivity and alters dopamine-related gene expression in larval zebrafish (Danio rerio).

摘要: Abstract Butylated hydroxytoluene (BHT) is one of the most frequently used synthetic phenolic antioxidants added to food and consumer products such as plastics a preservative. Due its high production volume, BHT has been detected in aquatic environments, raising concerns about sub-lethal toxicity. However, there are limited toxicological data for BHT, especially fish. In this study, zebrafish embryos were exposed at concentrations ranging 0.01–100 μM up 6 days post fertilization (dpf). Acute toxicity was assessed, experiments revealed that had 96 h LC50 value 57.61 μM. At doses (0.1–60 μM), markedly decreased heart rates 48 h 72 h by ∼25–30%. Basal maximal respiration 24 hpf 59.3% 41.4% respectively following exposure 100 μM BHT. Behavior measured dpf exposures 0.01–10 μM Locomotor behaviors (e.g. total distance moved velocity) significantly increased larvae higher than 0.1 μM addition, dark-avoidance behavior 0.01 μM while conversely, it To investigate potential underlying mechanisms could explain behavioral changes, transcripts involved dopamine signaling measured. Relative expression dat mRNA larval fish from treatment, no effects on levels concentrations. The drd3 1 μM treatment. Taken together, can affect system, which hypothesized be related abnormal anxiety-associated zebrafish.