Bone marrow origin of myofibroblasts in irradiation pulmonary fibrosis.

摘要: There is a rapid onset of organizing alveolitis/fibrosis at 120–140 d after whole lung irradiation C57BL/6J mice. To test the hypothesis that circulating cells bone marrow origin contribute to fibrosis, irradiated chimeric green fluorescent protein (GFP)+ mice were followed for GFP+ in areas fibrosis. In second experimental model, female received 20 Gy total irradiation, and 60 or 80 intravenously injected with from clonal male stromal cell line marrow, respectively. The then development pulmonary contribution Y-probe–positive, fibrotic was quantitated. Bromodeoxyuridine labeling developing showed division occurred predominantly GFP+, vimentin-positive cells. Immunohistochemistry demonstrated these macrophages fibroblasts, not endothelial...