Phenotypic characterization and differentiation potential of cells from human placenta: new approaches of stem cell theraphy for cystic fibrosis

摘要: Cystic fibrosis (CF) is the most frequent severe autosomal recessive disorder in European population. The gene located on long arm of chromosome 7 and encodes for CFTR protein (Cystic Fibrosis Transmembrane Conductance Regulator). Despite disease involves different organs, clinically relevant symptoms are found lung. Recently, given monogenic nature CF, therapy strategies have been developed, but results show that correction defect only temporary. Therefore, a novel approach based stem cell-based reconstitution airway epithelium expression should be explored. CF potential model cell because persistent lung inflammation leads to damage remodeling, can promote engraftment cells. If cells, transferred through autologous or heterologous transplant, would harbour niches respiratory epithelium, then restoration genetic cellular will definitive. In last years, several populations derived from adult bone marrow, amniotic fluid umbilical cord blood, shown differentiate epithelia, including respiratory. Even if vivo efficiency marrow cells very low, recent studies demonstrate small percentage corrected needed revert defect. Bone mesenchymal not without drawbacks, e.g. they limited numbers lack well-defined markers required their purification. One overcome these limitations consider alternative sources capable repopulating damaged epithelium. In present study, we evaluated possibility obtaining an ethically acceptable source as human placenta. As application CF therapy, placenta has advantage being natural by-product birth which often simply discarded, while harvest term membrane does pose any risk mother newborn. We demonstrated fetal tissue rich with characteristics capacity remain “plastic” differentiation options. Furthermore, display features low immunogenicity non tumorigenicity. For first time, our provided evidence epithelial (hAECs) mesenchimal stromal (hAMSCs) induced vitro express phenotype, like ZO-1, particularly when co-cultivated bronchial so into cells. Overall, data showed may contribute partial phenotype likely ideal candidate CF. Whether beneficial effects placenta-derived due transplanted themselves paracrine actions surrounding host order reduce regeneration remains fully elucidated. In case, promising obtained date constitute compelling regarding utility clinical application. Future testing model, help finding strategy cure directly transferable patients.