Down-Regulated Expression of A Disintegrin and Metalloproteinase with Thrombospondin-Like Repeats-1 by Progesterone Receptor Antagonist Is Associated with Impaired Expansion of Porcine Cumulus-Oocyte Complexes

摘要: ADAMTS-1, a member of the A disintegrin and metalloproteinase family proteases, is expressed in rodent follicles via progesterone receptor (PR)-dependent pathways. However, functional relationship between ADAMTS-1 expression PR has not been studied extensively other species. In present study, we investigated time-dependent changes cumulus cells porcine cumulus-oocyte complexes (COCs), roles expansion during vitro maturation oocytes. message was detected at time collection from follicles. response to gonadotropins, mRNA dramatically up-regulated reached maximum 20 h. The level mature protein increased manner with 40 induction significantly decreased by addition antagonist RU486 cultures. did affect ADAMTS-4 or factors that had reported be required for COC (TSG-6, versican, HA synthase-2). COCs cultured FSH LH h exhibited prominent expansion. reduced either Galardin, broad-spectrum matrix inhibitor. These results suggest through receptor-mediated action one essential requirements gonadotropin-regulated COCs.