Basic fibroblast growth factor: a potential regulator of proliferation and intermediate filament expression in the retina.

摘要: Proliferation of astrocytes, and a concomitant increase intermediate filaments in astrocytes are two fundamental responses the CNS to injury. We have previously identified these events retina's response detachment neural retina from adjoining monolayer retinal pigmented epithelium. In order analyze potential role basic fibroblast growth factor (bFGF) responses, we studied cellular proliferation filament protein expression retinas cats rabbits 4 d weeks after single intravitreal injection 1 microgram bFGF. Our results show that bFGF stimulates both processes an otherwise normal eye. The eyes received had significantly elevated numbers 3H-thymidine-labeled Muller cells, vascular epithelial microglia, macrophages by comparison control eyes. This was apparent at but not weeks. eyes, antibodies glial fibrillary acidic vimentin labeled only inner (vitread) portion specialized radial span width retina. been injected with bFGF, almost entire cell cytoplasm injection; weeks, cytoplasmic labeling intensity increased significantly. Release or activation endogenous stores injury disease may be involved elsewhere CNS.