Basic fibroblast growth factor stimulates 3H-thymidine uptake in retinal venular and capillary endothelial cells in vivo.

摘要: Recent studies have found basic fibroblast growth factor (bFGF), an angiogenic peptide, in retina and suggested that bFGF is responsible for retinal vascular proliferation. To test the hypothesis stimulates 3H-thymidine uptake cells vivo, we injected (100 ng) into vitreous cavity of six cats at 0 hr again 24 hr. Eight control eyes received boiled or no injection. After 46 hr, was all 2 later were enucleated. Intense seen with (56 +/- 20 SD positive per section) but not (7-10 section (P less than 0.001). Trypsin digest preparations showed thymidine predominantly venular (89%) capillary (10%) endothelium arterioles (1%) The data suggest endothelial respond preferentially to exogenous bFGF, part may explain their prominent role neovascular process. In a second group experiments ischemia releases diffusable similar can cause cells, created branch vein occlusion cat eyes. fellow injections. there intense within distribution occluded (84 77 section), none areas outside 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)