Regulation of new blood vessel growth into ischemic skeletal muscle

摘要: Abstract Purpose: In a rabbit model, transposition of muscle pedicle flap to an ischemic hind limb has been shown result in the development new blood vessels that connect arterial circulation limb. The hypothesis exogenous recombinant basic fibroblast growth factor (bFGF) would enhance this supply was examined and regulation bFGF process investigated. Methods: right common iliac artery ligated 12 male New Zealand white rabbits. An abdominal wall based on left inferior epigastric transposed thigh. phosphate-buffered saline (PBS) at 3 ng/h (n = 6), or PBS alone infused for 7 days via mini-osmotic pumps with infusion catheter positioned flap-muscle interface. interface immunostained anti-α–actin antibody determine vessel density (number vessels/mm) anti-bFGF evaluate distribution. RNA isolated from these sections, polymerase chain reaction (PCR) used examine endogenous messenger (mRNA) expression. Results: Blood significantly increased animals receiving (22.0 ± 10.6 vessels/mm vs. 10.7 8.8 vessels/mm, P .009). controls, neovessels were arranged clusters concentrated around clusters. bFGF-treated animals, diffusely scattered throughout flap-limb interface, corresponding distribution pattern bFGF. There no difference mRNA expression between control groups. Conclusion: Exogenous augmented Endogenous up-regulated newly developed microvessels correlated diffuse bFGF, implicating as important angiogenesis. did not affect expression, suggesting is under autocrine control. (J Vasc Surg 1998;28:919-28.)