Three Distinct Domains Contribute to Nuclear Transport of Murine Foxp3

摘要: Foxp3, a 47-kDa transcription factor, is necessary for the function of CD4+CD25+ regulatory T cells (Tregs), with an essential role in control self-reactive and preventing autoimmunity. Activation Tregs by TCR engagement results upregulation Foxp3 expression, followed its rapid nuclear transport binding to chromatin. Here, we identify three distinct domains that contribute transport. The first domain (Domain 1) comprises C-terminal 12 amino acids. second 2) located immediately N-terminal forkhead (FHD), recently reported be site runt-related factor 1/acute myeloid leukemia 1 (Runx1/AML1). third 3) within 51 Unlike known localization signals (NLSs), none these regions are rich basic residues do not bear any similarity monopartite or bipartite NLSs have one more clusters arginine-lysine-lysine-arginine (RKKR) sequence, 12-aa from end was previously signal (NLS) several proteins, including GFP-Foxp3 hybrid. Evidence provided here full-length native RKKR does as NLS. data this study indicates achieves other factors co-transporting them nucleus.