Homologous Recombination in Embryonic Stem Cells as a Means to Generate Mice with Defined Mutations

摘要: Publisher Summary This chapter presents experiments that employ a new method to mutate the mouse homeobox Hox-1.1 gene in ES cells. does not require selection and should allow introduction of small mutations without further alteration target locus. All mammalian Hox genes are organized clusters. These clusters very similar Drosophila antennapedia Bithorax their organization pattern expression. clustered might be important for regulation cis regulatory elements control expression several genes. It has been shown overexpression or ectopie interferes with normal embryonic development. For this reason, it was matter concern disruption neo r gene, which would introduce strong promoter into Hox-1 cluster, lead disregulation other cluster. make proper analysis − phenotype impossible. Thus, decided disrupt coding region by integrating 20-basepair (bp) oligonucleotide homeobox.