Methods to assess tissue permeability.

摘要: An essential requirement for adequate organ performance is the formation of permeability barriers that separate and maintain compartments distinctive structure function. The endothelial cell lining vasculature defines a semipermeable barrier between blood interstitial spaces all organs. Disruption can result in increased vascular leak. These effects are associated with multiple systemic disease processes accompany acute tissue responses to injury. mechanisms control function complex their full understanding requires multidisciplinary approach. use vivo data often complements molecular findings adds power studies. interaction types tissues present only mammalian models allows testing hypothesis establishing physiological significance results. In this chapter we describe simple methods be used systematically measure profile several