High levels of fish oil enhance neutrophil development and activation and influence colon mucus barrier function in a genetically susceptible mouse model

摘要: Dietary fatty acids influence immunologic homeostasis, but their effect on initiation of colitis, an immune-mediated disease, is not well established. Previously, our laboratory demonstrated that high doses dietary fish oil (FO) increased colon inflammation and dysplasia in a model infection-induced colitis. In the current study, we assessed effects high-dose FO, 6% by weight, inflammation, neutrophil recruitment function, mucus layer integrity genetically susceptible, colitis-prone mouse absence infection. FO-fed SMAD3(-/-) mice had evidenced numbers systemic local neutrophils chemoattractant inflammatory cytokine gene expression colon. Mucus thickness cecum goblet cell were reduced compared to control. FO consumption affected colitis male female differently. Compared control mice, from reactive oxygen species (ROS) upon ex vivo stimulation with phorbol myristate acetate while produced ROS control-fed mice. summary, impaired was associated characterized altered function. High-dose may have detrimental populations susceptible for bowel disease these differ between males females.