Comprehensive comparison of neonate and adult human platelet transcriptomes

摘要: Understanding the underlying mechanisms of well-substantiated platelet hyporeactivity in neonates is interest given their implications for clinical management newborns, a population at higher bleeding risk than adults (especially sick and preterm infants), as well gaining insight into regulatory biology. Transcriptome analysis useful identifying mRNA signatures affecting function. However, human fetal/neonatal transcriptome has never before been reported. We have used expression array first time to compare changes during development. Microarray was performed pure RNA obtained from adult cord blood, using same platform two independent laboratories. A high correlation between results both neonate samples. There also good agreement our samples outcomes previously reported three different studies. Gene enrichment showed that immunity- function-related genes are highly expressed developmental stages. Remarkably, 201 were found be differentially throughout In particular, neonatal platelets contain levels associated with protein synthesis processing, while carrying significantly lower involved calcium transport/metabolism cell signaling (including GNAZ). Overall, point variations possibly underlining hypo-functional phenotype provide further support role cellular immune response. Better characterization development can contribute elucidate how impact pathological conditions.