EGY1 encodes a membrane-associated and ATP-independent metalloprotease that is required for chloroplast development.

摘要: *Summary Chloroplast development requires coordinated expression of both nuclear- and chloroplast-encoded genes. To better understand the roles played by nuclear-encoded chloroplast proteins in biogenesis, we isolated an Arabidopsis mutant, egy1-1, which has a dual phenotype, reduced chlorophyll accumulation abnormal hypocotyl gravicurvature. Subsequent map-based cloning DNA sequencing mutant gene revealed 10-bp deletion EGY1 gene, encodes 59-kDa metalloprotease that contains eight transmembrane domains at its C-terminus, carries out b-casein degradation ATP-independent manner. protein varies between tissue types, being most prominent leaf stem tissues, is responsive to light ethylene. EGY1-GFP hybrid are localized chloroplast. egy1 chloroplasts had granal thylakoids poorly developed lamellae networks. Furthermore, a/b binding light-harvesting complexes I II (Lhca Lhcb) significantly decreased three separate loss-of-function mutants. Taken together, these results suggest required for and, hence, defective pleiotropic effects on ethylene-dependent gravitropism light-grown hypocotyls.