Fluorescence in situ hybridization confirms clearance of visible Helicobacter spp. associated with gastritis in dogs and cats.

摘要: Background: The results of studies examining the role Helicobacter spp. in pathogenesis canine and feline gastritis are inconclusive. Furthermore, data evaluating effectiveness medical therapy for eradication infection limited. Aim: To detect mucosal biopsies dogs cats diagnosed with gastritis, fluorescence situ hybridization (FISH). Animals: Three 2 signs chronic gastrointestinal disease. Methods: Dogs infected were treated triple antimicrobial fed an elimination diet 21 days. status endoscopic (3 dogs, 1 cat) or surgical (1 gastric mucosa was compared pre- posttreatment each animal by histology, FISH analysis, polymerase chain reaction (PCR). Results: Gastritis varying severity intraglandular spiral bacteria observed all animals. Pretreatment diagnostic tests confirmed presence animals histopathology 4/5 PCR. Rapid resolution vomiting episodes Gastric performed after revealed clearance visible negative as well PCR animals. Conclusions Clinical Importance: Application to routine biopsy specimens enabled rapid specific identification within cats. Although useful clinical biopsies, inflammation persisted.