Development of a novel microarray methodology for the study of SNPs in the promoter region of the TNF-α gene—Their association with obstructive pulmonary disease in Greek patients
作者： Athanasios Papatheodorou , Panagiota Latsi , Christina Vrettou , Aikaterini Dimakou , Alexia Chroneou
DOI: 10.1016/J.CLINBIOCHEM.2007.03.024
关键词:
摘要: Abstract Objectives: Polymorphisms in promoter region of TNF-α gene were shown to interfere with the transcriptional activity resulting production different levels product suggesting their involvement susceptibility or severity many inflammatory diseases. We set up a case–control study consisting 117 COPD (Chronic Obstructive Pulmonary Disease), 62 DB (bronchiectasis) patients and two control groups (109 smokers without COPD-healthy group 212 general population subjects) evaluate polymorphisms abovementioned diseases homogeneous population. Methods: The novel methodology NanoChip™ Molecular Biology Workstation (MBW Nanogen http://www.nanogen.com ) was employed genotype 5 SNPs. Results conclusions: Genotype frequencies SNPs showed no significant difference between patient healthy group. Statistical ( p = 0.043) only revealed haplotype compared MBW is an accurate method for SNP screening.
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