Identification of genetic variants in the TNF promoter associated with COPD secondary to tobacco smoking and its severity.

摘要: Chronic obstructive pulmonary disease (COPD) is an inflammatory that arises in response to noxious particles or gases. Associations of genetic polymorphisms TNF have been reported Asians and Caucasians, but not Mestizo populations. A case-control study was conducted two stages: the first stage, patients with COPD (COPD group, n=165) smokers without (SNC were included promoter sequence determined using direct sequencing. In second identified validated by real-time polymerase chain reaction (PCR) (n=260) SNC (n=506). 11 different sets "contig" alignments determined, which contig 10 found be associated susceptibility (P=5.0E-04, OR [odds ratio] =3.64) 1 Global Initiative for (GOLD) greater grade (P=1.0E-02, =3.82). The single nucleotide this region individually identified; GA genotypes rs1800629 (P=0.038, =2.07), rs56036015 (P=0.0082, =3.18), rs361525 =4.220) higher group vs group; after second-stage validation, (P=6.00E-03, =2.26) (P=1.10E-03, =2.54) are maintained. There variants increased risk secondary smoking a GOLD Mexican population.