Tumor Necrosis Factor–α rs361525 Polymorphism Is Associated with Increased Local Production and Downstream Inflammation in Chronic Obstructive Pulmonary Disease

摘要: Rationale: Chronic obstructive pulmonary disease (COPD) has a genetic component, explaining susceptibility. Tumor necrosis factor (TNF)-α polymorphisms have been associated with COPD, but it is unclear if genotype influences clinical phenotype, protein expression, and bioactivity.Objectives: To determine functional polymorphism was important by assessing TNF-α expression activity its association severity over time.Methods: Patients COPD rs361525 were matched to patients without polymorphism. TNF-α, antagonists, downstream mediators measured in plasma sputum. bioactivity, IL-8 secretion from primary bronchial epithelial cells (PBECs) measured, neutrophil migration assessed using sputum both subject groups the presence absence of antibody. Subjects followed annually compared.Measurements Main Results: had more chronic bronchitis, low...