Measles virus-specific human T cell clones. Characterization of specificity and function of CD4+ helper/cytotoxic and CD8+ cytotoxic T cell clones.

摘要: PBMC from healthy adult individuals seropositive for measles virus (MV) were tested their capacity to proliferate UV-inactivated MV (UV-MV) or autologous MV-infected EBV-transformed B cell lines (EBV-BC). MV-specific T responses observed in 11 of 15 donors (stimulation index greater than 2), when optimal doses UV-MV used proliferative assays. clones generated three responding MV, by using either EBV-BC as APC. Stimulation with exclusively CD3+ CD4+ CD8- cells, whereas after stimulation EBV-BC, both and CD4- CD8+ obtained. Of 19 so far, 7 reacted specifically purified fusion protein 1 hemagglutinin protein. Seven proliferated response the internal proteins MV. Three whole but not one proteins, clone seemed recognize more polypeptide. Some clones, vitro UV-MV, failed Ag APC instead PBMC. recognized association HLA class II (HLA-DQ -DR), most them displayed CTL activity EBV-BC. All II-restricted thus far capable assisting lymphocytes production antibody. The MARO I molecules cytotoxic toward