Expression of Factor VIII by Murine Liver Sinusoidal Endothelial Cells

摘要: Factor VIII (fVIII) is the procoagulant plasma glycoprotein that missing or decreased in hemophilia A. The cellular origin of fVIII synthesis controversial. Liver transplantation cures A, demonstrating liver a major site synthesis. We detected mRNA purified populations murine sinusoidal endothelial cells (LSECs) and hepatocytes, but not Kupffer cells. LSECs hepatocytes contained comparable numbers (40 70 transcripts per cell, respectively) by quantitative competitive reverse transcriptase-polymerase chain reaction analysis. There was detectable for factor IX, hepatocyte marker, LSEC preparation, nor there von Willebrand factor, an cell preparation. This excludes possibility due to cross-contamination preparations. Primary cultures were established which levels indistinguishable from LSECs. secreted active into culture medium. finding represents first demonstration homologous expression protein should facilitate studies gene regulation. Additionally, potentially are targets transgene during therapy