The HY2 gene as an efficient marker for transposon excision in Arabidopsis.

摘要: Transposable elements can generate germinal and somatic mutations, hence represent a powerful tool for the analysis of gene function. Transposons from maize have been adapted to mutagenise genomes diverse species. The efficiency these systems partly relies on ease with which (i.e. germinally transmitted) or excisions be detected. Here we describe use HY2, that codes an enzyme involved in biosynthesis phytochrome chromophore, monitor excision Ds gene-trap element Arabidopsis thaliana. Taking advantage altered germination de-etiolation behaviour -tagged hy2 mutant, designed efficient protocol recovery revertants, making HY2 most precocious marker available, best our knowledge. In addition, is also useful generating visible sectors photosynthetic tissues, thanks instability this mutable allele.