In Vivo toxicological assessment of biologically synthesized silver nanoparticles in adult Zebrafish (Danio rerio).
作者： Chandran Krishnaraj , Stacey L Harper , Soon-Il Yun
DOI: 10.1016/J.JHAZMAT.2015.09.022
关键词:
摘要: The present study examines the deleterious effect of biologically synthesized silver nanoparticles in adult zebrafish. Silver (AgNPs) used were by treating AgNO3 with aqueous leaves extract Malva crispa Linn., a medicinal herb as source reductants. LC50 concentration AgNPs at 96 h was observed 142.2 μg/l. In order to explore underlying toxicity mechanisms AgNPs, half (71.1 μg/l) exposed zebrafish for 14 days. Cytological changes and intrahepatic localization gills liver tissues respectively, results concluded possible sign oxidative stress. addition stress genotoxic peripheral blood cells like presence micronuclei, nuclear abnormalities also loss cell contact irregular shape parenchyma cells. Hence confirm effects mRNA expression related (MTF-1, HSP70) immune response (TLR4, NFKB, IL1B, CEBP, TRF, TLR22) genes analyzed clearly that plant mediated synthesis leads immunotoxicity
sci-hub.se 参考文章(51)
John Kiernan, Histological and Histochemical Methods: Theory and Practice ,(1981)
He T, Peng Ss, Zeng Lf, Hong, Wang Cj, Hou Yh, Zhang Xq, Huang Cy, In vivo and in vitro studies on the antitumor activities of MCP (Malva crispa L. Powder). Biomedical and Environmental Sciences. ,vol. 11, pp. 297- 306 ,(1998)
Monte Westerfield, The zebrafish book : a guide for the laboratory use of zebrafish (Danio rerio) M. Westerfield. ,(1995)
Robert J. Griffitt, Roxana Weil, Kelly A. Hyndman, Nancy D. Denslow, Kevin Powers, David Taylor, David S. Barber, Exposure to Copper Nanoparticles Causes Gill Injury and Acute Lethality in Zebrafish (Danio rerio) Environmental Science & Technology. ,vol. 41, pp. 8178- 8186 ,(2007) , 10.1021/ES071235E
Ji Eun Choi, Soohee Kim, Jin Hee Ahn, Pilju Youn, Jin Seok Kang, Kwangsik Park, Jongheop Yi, Doug-Young Ryu, Induction of oxidative stress and apoptosis by silver nanoparticles in the liver of adult zebrafish. Aquatic Toxicology. ,vol. 100, pp. 151- 159 ,(2010) , 10.1016/J.AQUATOX.2009.12.012
S.H. Doak, B. Manshian, G.J.S. Jenkins, N. Singh, In vitro genotoxicity testing strategy for nanomaterials and the adaptation of current OECD guidelines Mutation Research-genetic Toxicology and Environmental Mutagenesis. ,vol. 745, pp. 104- 111 ,(2012) , 10.1016/J.MRGENTOX.2011.09.013
Prakash M. Gopalakrishnan Nair, Jinhee Choi, Identification, characterization and expression profiles of Chironomus riparius glutathione S-transferase (GST) genes in response to cadmium and silver nanoparticles exposure. Aquatic Toxicology. ,vol. 101, pp. 550- 560 ,(2011) , 10.1016/J.AQUATOX.2010.12.006
Biplab Sarkar, Surya Prakash Netam, Arabinda Mahanty, Ashis Saha, Ranadhir Bosu, K. K. Krishnani, Toxicity Evaluation of Chemically and Plant Derived Silver Nanoparticles on Zebrafish ( Danio rerio ) Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. ,vol. 84, pp. 885- 892 ,(2014) , 10.1007/S40011-013-0298-Z
J. A. Heddle, M. C. Cimino, M. Hayashi, F. Romagna, M. D. Shelby, J. D. Tucker, Ph. Vanparys, J. T. MacGregor, Micronuclei as an index of cytogenetic damage: Past, present, and future Environmental and Molecular Mutagenesis. ,vol. 18, pp. 277- 291 ,(1991) , 10.1002/EM.2850180414
Shruti Pavagadhi, Muthuswamy Sathishkumar, Rajasekhar Balasubramanian, Uptake of Ag and TiO2 nanoparticles by zebrafish embryos in the presence of other contaminants in the aquatic environment Water Research. ,vol. 55, pp. 280- 291 ,(2014) , 10.1016/J.WATRES.2014.02.036