Effects of Dechlorane Plus exposure on axonal growth, musculature and motor behavior in embryo-larval zebrafish

摘要: Developmental neurobehavioral toxicity of Dechlorane Plus (DP) was investigated using the embryo-larval stages zebrafish (Danio rerio). Normal fertilized embryos were waterborne exposed to DP at 15, 30, 60 μg/L beginning from 6 h post-fertilization (hpf). Larval teratology, motor activity, motoneuron axonal growth and muscle morphology assessed different developmental stages. Results showed that exposure significantly altered embryonic spontaneous movement, reduced touch-induced movement free-swimming speed decreased swimming larvae in response dark stimulation. These changes occurred doses resulted no significant teratogenesis zebrafish. Interestingly, accord with these behavioral anomalies, inhibited primary induced apoptotic cell death lesions fibers Furthermore, 30 μg/L increased reactive oxygen species (ROS) malondialdehyde (MDA) formation, as well mRNA transcript levels apoptosis-related genes bax caspase-3. Together, our data indicate deficits may result combined effects neuronal connectivity injuries.