Somatic embryogenesis from floral tissues of feijoa (Feijoa sellowiana Berg)
作者： Suzana Stefanello , Lirio Luiz Dal Vesco , Jean Pierre H.J. Ducroquet , Rubens Onofre Nodari , Miguel Pedro Guerra
DOI: 10.1016/J.SCIENTA.2004.11.006
关键词:
摘要: Abstract The objectives of the present work were to study embryogenic competence floral tissues Feijoa sellowiana and investigate influence plant growth regulators on somatic embryo induction development in order establish a embryogenesis protocol starting from tissues. Petals, stamens ovaries buds cultivated onto LPm basal medium supplemented with different levels 2,4-D, Picloram, 2-iP, Kin BAP. highest callus was obtained Picloram (10 μM) (1 μM). Rates calluses petals significantly affected by PGRs. Embryogenic transferred same medium, gradually reduced PGRs-free medium. After 60 days suspension cultures 2,4-D (1 μM) 2-iP PGR-free 30 it observed globular embryos surface 18% friable previously induced Only derived gave rise this morphogenetic pattern.Torpedo cotyledonary culture converted complete plantlets. This is first report species
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