Isolation and characterization of simian immunodeficiency virus from mandrills in Africa and its relationship to other human and simian immunodeficiency viruses.

摘要: Two isolates of simian retrovirus related to the human immunodeficiency virus (HIV) were obtained from apparently healthy mandrills, Papio (Mandrillus) sphinx, in western equatorial Africa. This virus, designated SIVMND (simian mandrills), appeared morphologically similar HIV by electron microscopy, showed Mg2+-dependent reverse transcriptase activity, and induced cytopathic effect CD4-positive cells. Western blotting (immunoblotting) analyses revealed that gag pol products cross-reactivity with those known HIVs SIVs. Molecular clones covering full-length viral DNA closed circular extrachromosomal SIVMND-infected By clone-on-clone hybridization retroviruses SIV groups, cross-hybridization HIV-1, HIV-2, SIVAGM (African green monkey-derived SIV), SIVMAC (rhesus macaque-derived SIV) regions only at low stringency but not high stringency, a result indicating is new member HIV-SIV group. The existence distinct SIVs different monkey species suggest recent interspecies transfer unlikely nature.