作者: Jian Wang , Daniel Leung
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摘要: A simple confirmatory method for the determination of spiramycin and its metabolite neospiramycin in raw milk using LC ESI MS/MS is presented. Macrolide residues were extracted by ACN, sample extracts further cleaned up concentrated SPE cartridges. Both protonated electrospray positive ion mode to form singly and/or doubly charged pseudomolecular ions. Data acquisition was achieved multiple reaction monitoring, i.e., two transitions, quantification confirmation. Matrix-matched standard calibration curves utilized achieve best accuracy method. The performance evaluated according both a conventional validation procedure designed experimental result. measurement uncertainty arising from precision estimated. accuracy, expressed as percentage an overall recovery, 82.1 108.8%, intermediate less than 20%. LC/ESI-MS/MS LODs (S/N greater, not dbl equals 3:1) 1.0 microg/kg.