Separation analysis of macrolide antibiotics with good performance on a positively charged C18HCE column

摘要: The separation of basic macrolide antibiotics suffers from peak tailing and poor efficiency on traditional silica-based reversed-phase liquid chromatography columns. In this work, a C18HCE column with positively charged surface was applied to the macrolides. Compared an Acquity BEH C18 column, exhibited superior performance in aspect shape efficiency. screening mobile phase additives including formic acid, acetic acid ammonium formate indicated that preferable for providing symmetrical shapes. Moreover, influence content investigated. Analysis speed mass spectrometry compatibility were also taken into account when optimizing conditions coupled tandem spectrometry. developed method successfully utilized determination residues honey sample. Azithromycin chosen as internal standard quantitation spiramycin tilmicosin, while roxithromycin used erythromycin, tylosin, clarithromycin, josamycin acetylisovaleryltylosin. Good correlation coefficients (r(2) > 0.9938) all macrolides obtained. intra-day inter-day recoveries 73.7-134.7% 80.7-119.7% relative deviations 2.5-8.0% 3.9-16.1%, respectively. Outstanding sensitivity limits (S/N ≥ 10) 0.02-1 μg/kg detection 3) 0.01-0.5 achieved.