Soluble factor from tumor cells induces heme oxygenase-1 by a nitric oxide-independent mechanism in murine peritoneal macrophages

摘要: Tumor target-derived soluble secretary factor has been known to influence macrophage activation induce nitric oxide (NO) production. Since heme oxigenase-1 (HO-1) is induced by a variety of conditions associated with oxidative stress, we questioned whether from tumor cells induces HO-1 through NO-dependent mechanism in macrophages. We designated this as tumor-derived macrophage-activating (TMAF), because its ability activate macrophages iNOS. Although TMAF alone showed modest activity, combination IFN-γ significantly iNOS expression and NO synthesis. Simultaneously, induction was slightly augmented IFN-γ. Surprisingly, however, not inhibited the treatment highly selective inhibitor, 1400 W, indicating that enzyme NO-independent mechanism. While rIFN-γ iNOS, it had no effect on itself. Collectively, current study reveals target However, overall biological significance phenomenon remains be determined.