Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats.
作者： Rui Zeng , Jinmiao Li , Haijun Gong , Jiahao Luo , Zijing Li
DOI: 10.1155/2020/8206849
关键词:
摘要: The role of the IκB/NF-κB signaling pathway in uveoscleral outflow was investigated with IκBα gene silencing mediated by 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative. IκBα-siRNA-loaded DMAPA-Glyp complex transfected into ciliary muscles rats intracameral injection (labeled as DMAPA-Glyp+siRNA group). Lipofectamine™ 2000 (Lipo)/siRNA and naked siRNA were set controls. mRNA protein expression IκBα, NF-κBp65, MMP-2 analyzed real-time PCR, western blotting, situ gelatin zymography. Nuclear translocation NF-κBp65 immunofluorescence. Rat intraocular pressure (IOP) monitored pre- postinjection. Gene transfection efficiency toxicity derivative also evaluated. After RNA interference (RNAi), significantly inhibited. showed no significant differences. Nevertheless, nuclear occurred group. Both activity increased, largest increase IOP group fell to lowest level on day 3 after RNAi. levels Cy3-siRNA muscle did not decrease over time. At 7 14 d RNAi, pathological damage detectable eyes injected or DMAPA-Glyp/siRNA complex. Taken together, our results suggest that downregulation plays a crucial reducing values rats. may become new molecular target for lowering glaucoma. is safe feasible an effective vector rat eyes.
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