作者: Marta Pokrzywnicka , Robert Koncki , Łukasz Tymecki
DOI: 10.1007/S00216-014-8434-Z
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摘要: Integration of immobilized enzymes with light-emitting diodes (LEDs) leads to the development optoelectronic enzyme-based biosensors. In this work, urease, used as a model enzyme, in form of an open-tubular microbioreactor or biosensing membrane that has been integrated two red LEDs. It forms complete, fiberless, miniaturized, and extremely economic biooptoelectronic devices useful for nonstationary measurements under flow analysis conditions. Both biodevices, operating according to the paired emitter detector diode (PEDD) principle, allow relatively fast, highly sensitive, well-reproducible urea detection millimolar range concentrations. Potential analytical applications developed bioPEDDs have announced. presented constructions will be easily adapted other biosensors exploring various schemes biodetection.