Towards optoelectronic urea biosensors

作者: Marta Pokrzywnicka , Robert Koncki , Łukasz Tymecki

DOI: 10.1007/S00216-014-8434-Z

关键词:

摘要: Integration of immobilized enzymes with light-emitting diodes (LEDs) leads to the development optoelectronic enzyme-based biosensors. In this work, urease, used as a model enzyme, in form of an open-tubular microbioreactor or biosensing membrane that has been integrated two red LEDs. It forms complete, fiberless, miniaturized, and extremely economic biooptoelectronic devices useful for nonstationary measurements under flow analysis conditions. Both biodevices, operating according to the paired emitter detector diode (PEDD) principle, allow relatively fast, highly sensitive, well-reproducible urea detection millimolar range concentrations. Potential analytical applications developed bioPEDDs have announced. presented constructions will be easily adapted other biosensors exploring various schemes biodetection.

参考文章(50)
Robert Koncki, Marta Pokrzywnicka, Lukasz Tymecki, A very simple photometer based on paired-emitter-detector diodes Chemia Analityczna. ,vol. 54, pp. 427- 435 ,(2009)
King-Tong Lau, Susan Baldwin, Martina O’Toole, Roderick Shepherd, William J. Yerazunis, Shinichi Izuo, Satoshi Ueyama, Dermot Diamond, A low-cost optical sensing device based on paired emitter–detector light emitting diodes Analytica Chimica Acta. ,vol. 557, pp. 111- 116 ,(2006) , 10.1016/J.ACA.2005.10.046
Beata Rozum, Kamil Gajownik, Łukasz Tymecki, Robert Koncki, Poly(vinyl chloride) tubing with covalently bound alkaline phosphatase and alternative approach for investigations of open-tubular bioreactors Analytical Biochemistry. ,vol. 400, pp. 151- 153 ,(2010) , 10.1016/J.AB.2010.01.016
Phoonthawee Saetear, Kittiwut Khamtau, Nuanlaor Ratanawimarnwong, Kamonthip Sereenonchai, Duangjai Nacapricha, Sequential injection system for simultaneous determination of sucrose and phosphate in cola drinks using paired emitter-detector diode sensor Talanta. ,vol. 115, pp. 361- 366 ,(2013) , 10.1016/J.TALANTA.2013.05.051
I.M. Pérez de Vargas-Sansalvador, C. Fay, T. Phelan, M.D. Fernández-Ramos, L.F. Capitán-Vallvey, D. Diamond, F. Benito-Lopez, A new light emitting diode-light emitting diode portable carbon dioxide gas sensor based on an interchangeable membrane system for industrial applications. Analytica Chimica Acta. ,vol. 699, pp. 216- 222 ,(2011) , 10.1016/J.ACA.2011.05.019
Łukasz Tymecki, Kamil Strzelak, Robert Koncki, Biparametric multicommutated flow analysis system for determination of human serum phosphoesterase activity Analytica Chimica Acta. ,vol. 797, pp. 57- 63 ,(2013) , 10.1016/J.ACA.2013.08.047
Marta Pokrzywnicka, David J. Cocovi-Solberg, Manuel Miró, Víctor Cerdà, Robert Koncki, Łukasz Tymecki, Miniaturized optical chemosensor for flow-based assays Analytical and Bioanalytical Chemistry. ,vol. 399, pp. 1381- 1387 ,(2011) , 10.1007/S00216-010-4384-2
Robert Koncki, Analytical aspects of hemodialysis Trends in Analytical Chemistry. ,vol. 27, pp. 304- 314 ,(2008) , 10.1016/J.TRAC.2008.01.014
Kamil Strzelak, Agnieszka Wiśniewska, Dagna Bobilewicz, Robert Koncki, Multicommutated flow analysis system for determination of total protein in cerebrospinal fluid Talanta. ,vol. 128, pp. 38- 43 ,(2014) , 10.1016/J.TALANTA.2014.04.047