False labelling due to quenching failure of N‐hydroxy‐succinimide–ester‐coupled dyes

摘要: In comparative fluorescence gel electrophoresis experiments, cross-talk was detected. It traced back to a failure in the quenching process typical labelling protocols. Despite huge excess of potential reaction sites for N-hydroxy-succinimide-ester-coupled dye, sufficient active dye molecules were available after step label protein un-specifically. could be shown that only 100-fold increase amount quencher will silence residual such an extent no artificial signals are