Dissociated Neurons and Glial Cells Derived from Rat Inferior Colliculi after Digestion with Papain

摘要: The formation of gliosis around implant electrodes for deep brain stimulation impairs electrode–tissue interaction. Unspecific growth glial tissue the can be hindered by altering physicochemical material properties. However, in vitro screening neural tissue–material interaction requires an adequate cell culture system. No model cells dissociated from inferior colliculus (IC) has been described and was thus aim this study. Therefore, IC were isolated neonatal rats (P3_5) a established. In experiments using four dissociation methods (Neural Tissue Dissociation Kit [NTDK] T, NTDK P; PN, validated protocol spiral ganglion neurons [SGN]), optimal media, seeding densities identified. Thereafter, containing only proteolytic enzymes interest (trypsin or papain) tested. For analysis, fixed immunolabeled glial- neuron-specific antibodies. Adhesion survival demonstrated all experimental settings. Hence, preservation type-specific cytoarchitecture with sufficient neuronal networks occurred cultures P, fresh prepared papain solution. obtained after papain, seeded at density 2×104 cells/well cultivated Neuro Medium 6 days reliably revealed highest yield excellent cells. herein utilized as to screen interactions between surface modifications electrode.